Sodium Fluxes in Internally Dialyzed Squid Axons
نویسندگان
چکیده
منابع مشابه
Sodium Fluxes in Internally Dialyzed Squid Axons
The effects which alterations in the concentrations of internal sodium and high energy phosphate compounds had on the sodium influx and efflux of internally dialyzed squid axons were examined. Nine naturally occurring high energy phosphate compounds were ineffective in supporting significant sodium extrusion. These compounds were: AcP, PEP, G-3-P, ADP, AMP, GTP, CTP, PA, and UTP.(1) the compoun...
متن کاملSodium Extrusion by Internally Dialyzed Squid Axons
A method has been developed which allows a length of electrically excitable squid axon to be internally dialyzed against a continuously flowing solution of defined composition. Tests showed that diffusional exchange of small molecules in the axoplasm surrounding the dialysis tube occurred with a half-time of 2-5 min, and that protein does not cross the wall of the dialysis tube. The composition...
متن کاملSome Factors Affecting Sodium Outflux in Internally Dialyzed Squid Axons
Although ATP has been shown to be an energy source for sodium extrusion in injected squid axons (CaldweU, Hodgkin, Keynes, and Shaw, 1960) as well as dialyzed axons (Brinley and Mullins, 1967; Mullins and Brinley, 1967), the ability of other high-energy phosphate compounds to support sodium extrusion has not been thoroughly studied. An internal dialysis technique has been used for such a study ...
متن کاملSome Factors Influencing Sodium Extrusion by Internally Dialyzed Squid Axons
Squid giant axons were internally dialyzed by a technique previously described. In an axon exposed to cyanide seawater for 1 hr and dialyzed with an ATP-free medium, the Na efflux had a mean value of 1.3 pmole/cm(2)sec when [Na](i) was 88 mM, in quantitative agreement with flux ratio calculations for a purely passive Na movement. When ATP at a concentration of 5-10 mM was supplied to the axopla...
متن کاملIon Fluxes in Dialyzed Squid Axons
A method will be described which allows the control of internal solute composition in squid axons while they are engaging in metabolically dependent ion transport. A glass tube, about 250 m m long and 0.175 m m in diameter containing a central 20 m m long region of porous glass, was inserted into the axon, and the porous region was positioned in the middle of the axon. When solutions of a defin...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1968
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.52.2.181